Alan Attie

Alan Attie
Professor

Ph.D., University of California-San Diego, 1980
Postdoctoral Research: University of California-San Deigo, Department of Medicine 

Address: 534A Biochemistry Addn

Telephone: 608-262-1372

E-mail:

Department: Biochemistry

Research Interests

Genetics of type 2 diabetes and related metabolic diseases.

Research Fields

Disease Biology
Cell Biology
Computational, Systems & Synthetic Biology
Gene Expression
Genomics & Proteomics
Human, mouse & rat

Research Description:

Genetics of diabetes. Our laboratory uses mouse genetics to identify genes and pathways involved in obesity-induced type 2 diabetes. We have reproduced the obesity/diabetes dichotomy in mice by studying two strains that when made obese, differ in diabetes susceptibility. Using this model system, we have mapped several diabetes gene loci. We recently identified two genes underlying these loci. One of the genes affects insulin action and the other affects insulin secretion.

Gene causal networks and diabetes. Using microarray technology, we have identified genes whose expression changes before, during, and after the onset of diabetes. Many of these patterns are highly correlated, indicating coordinate regulation of networks of gene expression. These networks have control points, e.g. signaling molecules or transcription factors. We are identifying these points and testing their function in biological systems.

Molecular biology of ß-cell proliferation. We have identified several factors involved in stimulating ß-cell proliferation. We wish to discover the receptors and the signaling pathways involved in this critically important process.

The genetics of gene expression.
Traditional genetics correlates genotype with phenotype in a complex outbred population or in an experimental cross. This identifies areas of the genome controlling the phenotype of interest. We expand our definition of phenotype to include mRNA abundance on the large scale available through microarray technology. By mapping mRNA abundance, we map gene loci controlling the expression of many thousands of mRNA transcripts. These loci are termed expression quantitative trait loci (eQTL). With this approach, we are uncovering gene regulatory networks that are dysregulated in obesity and diabetes.

Micro-RNA regulation of insulin secretion. We have identified two miRNAs that stimulate insulin secretion. We are working to identify the targets of these miRNAs the mechanisms underlying their effect on insulin secretion.

Genetics of hepatic steatosis.
Hepatic steatosis (fatty liver) is the pathological accumulation of excess lipid (usually triglyceride) in hepatocytes. We have mapped a locus that affects this trait and identified two novel candidate genes. 

Representative Publications:

Search PubMed for more publications by Alan Attie

Kimple, M.E., Keller, M.P., Rabaglia, M.R., Pasker, R.L., Truchan, N.A., Neuman, J.C., Brar, H.K., and Attie, A.D. 2013. The prostaglandin E2 receptor, EP3, is induced in diabetic islets and negatively regulates glucose- and hormone-stimulated insulin secretion. Diabetes.

Tu, Z., Keller, M.P., Zhang, C., Rabaglia, M.E., Greenawalt, D.M., Yang, X., Wang, I.M., Dai, H., Bruss, M.D., Lum, P.Y., et al. 2012. Integrative analysis of a cross-loci regulation network identifies App as a gene regulating insulin secretion from pancreatic islets. PLoS Genet 8:e1003107. 

Wang, C.Y., Stapleton, D.S., Schueler, K.L., Rabaglia, M.E., Oler, A.T., Keller, M.P., Kendziorski, C.M., Broman, K.W., Yandell, B.S., Schadt, E.E., et al. 2012. Tsc2, a positional candidate gene underlying a quantitative trait locus for hepatic steatosis. J Lipid Res 53:1493-1501.

Bhatnagar, S., Oler, A.T., Rabaglia, M.E., Stapleton, D.S., Schueler, K.L., Truchan, N.A., Worzella, S.L., Stoehr, J.P., Clee, S.M., Yandell, B.S., et al. 2011. Positional cloning of a type 2 diabetes quantitative trait locus; tomosyn-2, a negative regulator of insulin secretion. PLoS Genet 7:e1002323.

Raines, S.M., Richards, O.C., Schneider, L.R., Schueler, K.L., Rabaglia, M.E., Oler, A.T., Stapleton, D.S., Genove, G., Dawson, J.A., Betsholtz, C., et al. 2011. Loss of PDGF-B activity increases hepatic vascular permeability and enhances insulin sensitivity. Am J Physiol Endocrinol Metab 301:E517-526.

Lavine, J.A., and Attie, A.D. 2010. Gastrointestinal hormones and the regulation of beta-cell mass. Ann N Y Acad Sci 1212:41-58.

Lane, R.F., Raines, S.M., Steele, J.W., Ehrlich, M.E., Lah, J.A., Small, S.A., Tanzi, R.E., Attie, A.D., and Gandy, S. 2010. Diabetes-associated SorCS1 regulates Alzheimer's amyloid-beta metabolism: evidence for involvement of SorL1 and the retromer complex. J Neurosci 30:13110-13115.

Babak, T., Garrett-Engele, P., Armour, C.D., Raymond, C.K., Keller, M.P., Chen, R., Rohl, C.A., Johnson, J.M., Attie, A.D., Fraser, H.B., et al. 2010. Genetic validation of whole-transcriptome sequencing for mapping expression affected by cis-regulatory variation. BMC Genomics 11:473.

Davis, D.B., Lavine, J.A., Suhonen, J.I., Krautkramer, K.A., Rabaglia, M.E., Sperger, J.M., Fernandez, L.A., Yandell, B.S., Keller, M.P., Wang, I.M., et al. 2010. FoxM1 is up-regulated by obesity and stimulates beta-cell proliferation. Mol Endocrinol 24:1822-1834.

Lavine, J.A., Raess, P.W., Stapleton, D.S., Rabaglia, M.E., Suhonen, J.I., Schueler, K.L., Koltes, J.E., Dawson, J.A., Yandell, B.S., Samuelson, L.C., et al. 2010. Cholecystokinin is up-regulated in obese mouse islets and expands beta-cell mass by increasing beta-cell survival. Endocrinology 151:3577-3588.

Keller, M.P., and Attie, A.D. 2010. Physiological insights gained from gene expression analysis in obesity and diabetes. Annu Rev Nutr 30:341-364.

Newgard, C.B., and Attie, A.D. 2010. Getting biological about the genetics of diabetes. Nat Med 16:388-391.