Ph.D., University of California, San Diego, 2002
Postdoctoral Research: Ludwig Institute for Cancer Research, La Jolla, California
Lab website: http://www.audhyalab.org
Address: 5214A Biochemical Sciences Bldg
Department: Biomolecular Chemistry
Research InterestsRegulation of vesicle biogenesis and membrane transport during development
Research FieldsDisease Biology
Genomics & Proteomics
Neuro & Behavioral Genetics
Human, mouse & rat
Our laboratory is committed to understanding the fundamental mechanisms by which membrane proteins, lipids, and other macromolecules are transported throughout eukaryotic cells. To do so, we take advantage of numerous interdisciplinary approaches, including genetics, biochemistry, structural biology, biophysics, molecular biology and high-resolution fluorescence and electron microscopy.
Additionally, we use a variety of experimental systems, ranging from simple animal models (e.g. Caenorhabditis elegans) to human induced pluripotent stem cells (iPSCs). We also aim to recapitulate individual steps of membrane transport in vitro, using recombinant proteins and chemically defined lipids. Our ultimate goal is to identify the regulatory pathways that control membrane deformation, which enable vesicle formation in the endosomal and secretory systems. Although basic research is the cornerstone of our program, we also seek to define pathomechanisms that underlie human disease, focusing on the impact of mutations in key trafficking components that lead to cancer, neurodegeneration, asthma, and diabetes.
Schuh, A.L., Hanna, M., Quinney, K., Wang, L., Sarkeshik, A., Yates, J.R., and Audhya, A. (2015) The VPS-20 Subunit of the Endosomal Sorting Complex ESCRT-III Exhibits an Open Conformation in the Absence of Upstream Activation. Biochem. J. 466: 625-637.
Johnson, A., Bhattacharya, N., Hanna, M., Pennington, J.G., Schuh, A.L., Wang, L., Otegui, M.S., Stagg, S.M., and Audhya, A. (2015) TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. EMBO J. 34: 811-827.
Takahashi, H., Mayers, J.R., Wang, L., Edwardson, J.M., and Audhya, A. (2015) Hrs and STAM function synergistically to bind ubiquitin-modified cargoes in vitro. Biophys. J. 108: 76-84.