Ph.D., University of Wisconsin-Madison
Postdoctoral Research: Developmental Biology, National Institutes of Health
Address: 353 Bardeen Labs
My laboratory's focus is development and disorders of the visual system using the mouse as the model system.
Human, mouse & rat
Cell adhesion and polarity are vital to the growth and
differentiation of epithelial tissues and to maintaining their long-term
integrity. We are interested in understanding the molecular and genetic
pathways that are responsible for establishing and maintaining epithelial
identity during development and how disruptions in these pathways contribute to
disease. In particular, we have focused our efforts on determining the
molecular pathways that regulate the development of various epithelial tissues
of the mouse eye. The mouse eye offers a number of advantages for study. It is
nonessential for animal viability and accessible yet the properties of the
epithelial tissues in this organ reflect the properties of epithelia throughout
the organism. We use a variety of genetic, molecular, cell biological and
embryological techniques and transgenic and knockout mice.
In invertebrates such as Drosophila melanogaster members of the PDZ (PSD95/Dlg/ZO-1) family such as dlg and scrib regulate cell adhesion, polarity and proliferation. A major emphasis at the present time in the Griep lab is to understand how the mouse homologs of these two genes, contribute to eye development. Our recent analysis suggests that Dlg-1 and Scrib may regulate lens cell growth and differentiation in part by affecting cell adhesion complexes and their associated signaling cascades. Furthermore, at least Scrib is required to maintain epithelial identity and both Dlg-1 and Scrib may affect planar cell polarity in the lens, a newly identified property of the lens that is thought to be essential for lens cell differentiation. Current and future work will continue to explore the specific roles of these proteins in mouse development and to uncover the mechanistic connection between growth factor receptor signaling, cell adhesion, polarity and cell cycle control, in the regulation of lens cell proliferation and differentiation.
Rivera, C., I.F. Yamben, S.
Shatadal, M.A. Waldof, M.L. Robinson, and A.E.
Griep. 2009. Cell autonomous requirements for Dlg-1 for lens epithelial cell structure and fiber cell
morphogenesis. Dev. Dyn. 238: 2292-2308.
Griep, A.E. 2006. Cell Cycle Regulation in the Developing Lens. Sem. Cell Dev. Biol. 17: 686-697.
Nguyen, M.M., C. Rivera, and A.E. Griep. 2005. Localization of PDZ domain containing proteins Discs large-1 and Scribble in the mouse eye. Mol. Vis. 11: 1183-1199.
Nguyen, M.M., M.L. Nguyen, G. Caruana, A. Bernstein, P.F. Lambert, and A.E. Griep. 2003. Requirement of PDZ domain containing proteins for cell cycle regulation and differentiation in the mouse lens epithelium. Mol. Cell. Biol. 23: 8970-8981.
Foley, J.D. H. Rosenbaum, and A.E. Griep. 2004. Temporal regulation of VEID-AFC cleavage activity and caspase 6 correlates with organelle loss during lens development. J. Biol. Chem. 279: 32142-32150.
Hyde, R.K. and A.E. Griep. 2002. Unique roles for E2f1 in the mouse lens in the absence of functional pRB proteins. Invest. Ophthalmol. Vis. Sci. 43: 1509-1516.
Stolen, C.M. and A.E. Griep. 2000. Disruption of lens fiber cell differentiation and survival at multiple stages by region-specific expression of truncated FGF receptors. Dev. Biol. 217: 205-220.
Pan, H. and A.E. Griep. 1995. Temporally distinct patterns of p53-dependent and p53-independent apoptosis during mouse lens development. Genes Dev. 9: 2157-2169.