Ph.D., M.I.T., 2006
Postdoctoral Research: University of California, Berkeley
Address: 6204B Biochemical Sciences Building
Department: Biomolecular Chemistry
Research InterestsWe are studying embryonic development to understand the transcriptional mechanisms required to establish a totipotent state.
Genomics & Proteomics
Development and differentiation are driven by coordinated changes
in gene expression. Early zygotic
development is controlled by maternally contributed mRNAs and proteins, and
transcriptional activation of the zygotic genome is delayed until hours after
fertilization. This delayed
transcriptional activation is a nearly universal phenomenon in all
metazoans. Immediately following
fertilization, the genome undergoes epigenetic reprogramming to allow for the
transition from a specified germ cell to the pluripotent cells of the early
embryo. The zygotic genome remains
transcriptionally quiescent during these initial stages. Only at later cell cycles is widespread
zygotic transcription initiated. This zygotic
genome activation is tightly coordinated with the degradation of maternally
provided mRNAs at the maternal-to-zygotic transition (MZT). Thus during this discrete developmental time
point the transcriptional profile of the developing embryo undergoes a
While most of the genome is silenced prior to the MZT, a small subset of genes is expressed. These genes control fundamental processes essential for the future development of the organism and allow for progression through the MZT. Despite the fact that these developmental events are common from plants to vertebrates, little is currently known about the factors that regulate zygotic genome activation at the MZT or the mechanisms that allow for the selective transcription of a handful of genes at earlier time points. An understanding of these mechanisms will provide insights into how the genomes of pluripotent cells in general, including embryonic, cancer and induced-pluripotent stem cells, maintain their broad developmental potential while being poised to differentiate.
We use Drosophila melanogaster to study these phenomena using a wide variety of tools including biochemistry, genetics, molecular biology, genomics, and cell biology. Broadly, we are seeking to understand three outstanding questions: (1) why most of the genome is not transcribed until many nuclear divisions after fertilization, (2) how a small number of genes are expressed when the remainder of the genome is not, and (3) what triggers the widespread activation of the zygotic genome at the MZT.
Hamm, D.C., Bondra, E.R., M.M. Harrison. (2015) Transcirptional activation is a conserved feature of the early embryonic factor Zelda that requires a cluster of four zinc fingers for DNA binding and a low-complexity activation domain. J Biol Chem. 290: 3508-3518.
Li, X.Y., Harrison, M.M., Villalta, J.E., Kaplan, T. and M.B. Eisen. (2014) Establishment of regions of genomic activity during the Drosophila maternal to zygotic transition. eLife.3: doi 10.7554/eLife.03737.
Harrison, M.M., Jenkins, B.V., O’Connor-Giles, K.M. and J. WIldonger. (2014) A CRISPR view of development. Genes Dev. 28: 1859-1872.
Gratz, S.J., Cummings, A.M., Nguyen, J.N., Hamm, D.C., Donohue, L.K., Harrison, M.M.*, Wildonger, J.* and K.M. O’Connor-Giles*. (2013) Genome engineering of Drosophila with the CRISPR RNA-guided Cas9-nuclease. Genetics. 194:1029-1035.
Harrison, M.M. *, Li, X.Y*. Kaplan, T. *, Botchan, M.R., and M.B. Eisen. (2011) Zelda Binding in the Early Drosophila melanogaster Embryo Marks Regions Subsequently Activated at the Maternal-to-Zygotic Transition. PLoS Genet. 7:e1002266.
Harrison, M.M., Botchan, M.R. and T.W. Cline. (2010) Grainyhead and Zelda compete for binding to the promoters of the earliest-expressed Drosophila genes. Dev Biol. 345: 248-255.