Jue D. (Jade) Wang
Ph.D., University of California-San Francisco, 2002
Postdoctoral Research: Massachusetts Institute of Technology
Lab website: http://www.bact.wisc.edu/faculty.php?init=JDW
Address: 6478 Microbial Sciences Building
Genome stability, DNA replication and stress response in Bacteria
Our research focuses on how living organisms accurately duplicate and process their genetic information by regulating central dogma processes- replication, transcription and translation. These processes lie at the heart of all cellular activities and are crucial for survival and genome stability. We study these processes in the Gram-positive bacterium Bacillus subtilis and the Gram-negative bacterium Escherichia coli. They have simpler modes of replication and transcription than eukaryotes and are highly amenable to genetic manipulation. We take a global approach, combining genetic, genomic, biochemical, and computational methods to uncover essential, previously unknown aspects of central dogma regulation: identifying a molecular interface between the DNA replication machinery and its cellular milieu; understanding the conflicts between replication and transcription; and discovering how evolutionarily divergent bacteria utilize a ubiquitous molecule for stress-resistance. Because the fundamentals of information-processing mechanisms are conserved across all domains of life, our work in bacteria is broadly applicable to other less-tractable systems.
Liu K, Myers AR, Pisithkul T, Claas KR, Satyshur KA, Amador-Noguez D, Keck JL, Wang JD. (2015) "Molecular mechanism and evolution of guanylate kinase regulation by (p)ppGpp." Molecular Cell. 57(4):735-49.
Zhang Y, Mooney RA, Grass JA, Sivaramakrishnan P, Herman C, Landick R, Wang JD. (2014) "DksA guards elongating RNA polymerase against ribosome-stalling-induced arrest." Molecular Cell. 53(5):766-78.
Kriel A, Bittner AN, Kim SH, Liu K, Tehranchi AK, Zou WY, Rendon S, Chen R, Tu BP, Wang JD. (2012) “Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance.” Molecular Cell. 48(2):231-41.
Tehranchi AK, Blankschien MD, Zhang Y, Halliday JA, Srivatsan A, Peng J, Herman C, Wang JD. (2010) “The transcription factor DksA prevents conflicts between DNA replication and transcription machinery.” Cell. 141(4):595-605.
Srivatsan A, Tehranchi A, MacAlpine DM, Wang JD. (2010) “Co-orientation of replication and transcription preserves genome integrity.” PLoS Genetics. 6(1):e1000810.