University of Wisconsin–Madison
College of Agriculture and Life Sciences | School of Medicine and Public Health

Aaron Hoskins

Assistant Professor

ahoskins@wisc.edu

608-890-3101

Biochemistry
RNA splicing, gene expression, fluorescence microscopy, RNA/protein complexes

Address
2214 Biochemical Sciences Building
Education
Ph.D., Massachusetts Institute of Technology, 2006
Department
Biochemistry
Research Interests
RNA splicing, gene expression, fluorescence microscopy, RNA/protein complexes, molecular mechanism, gene regulation, yeast genetics and biology
Research Fields
Biochemistry, Single-Molecule Biophysics, Yeast Genetics, Chemical Biology, Molecular Biology

Research Description:
My laboratory is interested in how a molecular machine called the spliceosome functions and is regulated during gene expression. In particular, we are interested in how the spliceosome identifies particular locations within introns before they are removed during pre-mRNA splicing. We use a combination of genetic, biochemical, and biophysical approaches to study the spliceosome in vitro and in cells.


Representative Publications:
Search PubMed for more publications by Aaron Hoskins

1. Larson, J.D., Kirk, M., O’Brien, W., MacKay, J.F., Friedman, L.J., and Hoskins, A.A. Design and construction of a multiwavelength micromirror total internal reflectance fluorescence microscope. Nat Protoc. v9 (2014), 2317-28. PMID: 25188633.

2. Rodgers, M.L., Paulson, J., and Hoskins, A.A. Rapid Isolation and Single molecule Analysis of Ribonucleoproteins from Cell Lysate by SNAP-SiMPull, RNA v21 (2015), 1031-41. PMCID: PMC4408783.

3. Xu, J., Carrocci, T.J., and Hoskins, A.A. † Evolution and Characterization of a benzylguanine-binding RNA aptamer. Chem Commun (Camb), v52 (2016), 549-52. PMID: 26538152. PMCID: In Process.

4. Rodgers, M.L. , Tretbar, S.U., DeHaven, A., Alwan, A.A., Luo, G., Mast, H.M., and Hoskins, A.A. Conformational Dynamics of Stem II of the U2 snRNA. RNA, v22 (2016), 225-36. PMCID: PMC4712673

5. Hoskins, A.A., Rodgers, M.L., Friedman, L.J., Gelles, J., and Moore, M.J. Single Molecule Analysis Reveals Reversible and Irreversible Steps during Spliceosome Activaiton. eLife, (2016), Accepted and In Press. http://dx.doi.org/10.7554/eLife.14166