Jae-hyuk Yu

Position title: Professor

Email: jyu1@wisc.edu

Phone: 608-262-4696

Molecular genetics and genomics of spore formation and mycotoxin biosynthesis in filamentous fungi

3155 Microbial Sciences Building
Ph.D., The University of Wisconsin-Madison (1995), Postdoctoral Research: Texas A&M University, 1995-1998; Investigator (Scientist): Cereon Genomics, LLC. 1998-2000
Research Interests
Molecular genetics and genomics of spore formation and mycotoxin biosynthesis in filamentous fungi
Research Fields
Development, Gene Expression, Genomics & Proteomics, Fungi

Research Description:
The genus Aspergillus encompasses the most common fungi in our environment. Many Aspergillus species are beneficial to humans, but they also include serious animal and plant pathogens. Moreover, most (if not all) Aspergillus species have the ability to produce one or more toxic secondary metabolites called mycotoxins. All Aspergilli produce asexual spores as the main means of dispersion and biosynthesis of certain mycotoxins is intimately related with fungal sporulation. The primary interest of my research program is to understand how fungi coordinate growth, sporulation and toxin biosynthesis employing the model fungus Aspergillus nidulans. We showed that two antagonistic regulatory pathways govern vegetative growth and sporulation in A. nidulans. Vegetative growth is primarily mediated by a heterotrimeric G protein, which stimulates fungal growth while inhibiting asexual/sexual sporulation as well as production of the carcinogenic mycotoxin sterigmatocystin. We found that the initiation, progression and completion of sporulation are directed by the balanced activities of multiple positive and negative regulators. We are further investigating the detailed molecular mechanisms regulating these fundamental biological processes via forward/reverse genetics, genomics and biochemical analyses.

Representative Publications:
Search PubMed for more publications by Jae-hyuk Yu

Park, H-S., Yu, Y.M., Lee, M-K., Maeng, P.J. Kim, S.C., and Yu, J.-H. 2015. Velvet-mediated repression of β-glucan synthesis in Aspergillus nidulans spores. Scientific Rep. 5:10199 | DOI: 10.1038/srep10199

Alkhayyat, F., Kim, S.C., and Yu, J.-H. 2015. Genetic control of asexual development in Aspergillus fumigatus, Advances in Applied Microbiology, 90: 93-107

Chen, W, Lee, M.-K., Jefcoate, C., Kim, S.C., Chen, F., and Yu, J.-H. 2014. Fungal cytochrome P450 monooxygenases: Their distribution, structure, functions, family expansion, and evolutionary origin. Genome Biology and Evolution. 6: 1620-1634.

Lee, M.-K., Kwon, N.-J., Choi, J.-M., Lee, I.-S., Jung, S. and Yu, J.-H., 2014. NsdD is a key repressor of asexual development in Aspergillus nidulans. Genetics, 197: 159-173 doi: 10.1534/genetics.114.161430

Park, H.-S., Nam, T.-Y., Han, K.-H., Kim, S.-C., and Yu, J.-H., 2014. VelC positively controls sexual development in Aspergillus nidulans. PLoS ONE, 9(2): e89883. doi:10.1371/journal.pone.0089883

Alkhayyat, F. and Yu, J.-H. 2014. Upstream Regulation of Mycotoxin Biosynthesis, Advances in Applied Microbiology, 86: 251-278 doi: 10.1016/B978-0-12-800262-9.00005-6 (Invited Review)

Ahmed, Y.L.*, Gerke, J.*, Park, H-S.*, Bayram, Ö, Neumann, P., Ni, M., Dickmanns, M., Kim, S.C., Yu, J.-H., Braus, G.H., Ficner, R. 2013. The Velvet family of fungal regulators contains a DNA-binding domain structurally similar to NF-kB. PLoS Biology 11(12): e1001750. doi:10.1371/journal.pbio.1001750 (* Equal contribution, Co-Corresponding Authors underlined) – Weekly Editors’ Pick.

Kong, Q., Long, W., Liu, Z., Kwon, N.-J., Kim, S.-C. and Yu, J.-H. 2013. Gbeta-like CpcB plays a crucial role for growth and development of Aspergillus nidulans and Aspergillus fumigatus. PLoS ONE 8(7): e70355. doi:10.1371/journal.pone.0070355

Shin, K.-S., Park, H.-S., Kim, Y.-H. and Yu, J.-H. 2013. Comparative proteomic analyses reveal that FlbA down-regulates gliT expression and SOD activity in Aspergillus fumigatus. J Proteomics 87:40-52. doi: 10.1016/j.jprot.2013.05.009.

Jeong, K.-C., and Yu, J.-H. 2012. Investigation of in vivo protein interactions in Aspergillus spores. Methods in Molecular Biology 944: 251-257. doi:10.1007/978-1-62703-122-6_18.

Park, H.-S., and Yu, J.-H. 2012. Multi-Copy Genetic Screen in Aspergillus nidulans. Methods in Molecular Biology 944: 183-190. doi:10.1007/978-1-62703-122-6_13.

Park, H-S., and Yu, J-H. 2012. Genetic control of asexual sporulation in filamentous fungi, Current Opinion in Microbiology, 15: 669-677. doi:10.1016/j.mib.2012.09.006 (Invited Review)

Kwon, N-J., Park, H-S., Jung, S., Kim, S.C., and Yu, J-H. 2012. The putative guanine nucleotide exchange factor RicA mediates upstream signaling for growth and development in Aspergillus. Eukaryotic Cell 11: 1399-1412

Park, H-S., Ni, M., Jeong, K-C., Kim, Y-H., and Yu, J-H. 2012. The role, interaction and regulation of the velvet regulator VelB in Aspergillus nidulans, PLoS ONE 7(9): e45935. doi:10.1371/journal.pone.0045935

Park, H-S., Bayram, Ö, Braus, G.H., Kim, S-C., and Yu, J-H. 2012. Characterization of the velvet regulators in Aspergillus fumigatus. Molecular Microbiology. 86: 937-953 DOI: 10.1111/mmi.12032

Bayram, Ö.S., Bayram, Ö, Valerius, O., Park, H.-S., Irniger S., Gerke, J., Ni, M., Han, K.-H., Yu, J.-H., and Braus, G.H. 2010. LaeA control of velvet family regulatory proteins for light-dependent development and fungal cell-type specificity. PLoS GENETICS, 6(12):e1001226.

Tao, L., and Yu, J.-H. 2011. AbaA and WetA govern distinct stages of Aspergillus fumigatus development. Microbiology-SGM, 157: 313 – 326. doi:10.1099/mic.0.044271-0 Yu, J.-H. 2010. Regulation of Development in Aspergillus nidulans and Aspergillus fumigatus. Mycobiology, 38: 229-237.

Xiao, P., Shin, K.-S., Wang, T., and Yu, J.-H. 2010. Aspergillus fumigatus flbB encodes two basic leucine zipper domain (bZIP) proteins required for proper asexual development and gliotoxin production. Eukaryotic Cell, 9: 1711-1723.

Kwon, N.-J., Shin, K.-S., and Yu, J-H. 2010. Characterization of the developmental regulator FlbE in Aspergillus fumigatus and Aspergillus nidulans. Fungal Genetics and Biology, 47: 981-993.

Kwon, N.-J., Garzia, A., Espeso, E.A., Ugalde, U., and Yu, J-H. 2010. FlbC is a putative nuclear C2H2 transcription factor regulating development in Aspergillus nidulans. Molecular Microbiology 77: 1203-1219.

Shin, K.-S., Kwon, N.-J., Park, H.-S., Kwon, G.-S., and Yu, J.-H. 2009. Differential roles of the ChiB chitinase in autolysis and cell death of Aspergillus nidulans. Eukaryotic Cell, 8:738-746.

Bayram, Ö, Krappmann, S., Ni, M., Bok, J.-W., Helmstaedt, K., Valerius, O., Braus-Stromeyer, S., Kwon, N-J., Keller, N.P., Yu, J.-H., and Braus, G.H. 2008. The velvet complex coordinates light, fungal development and secondary metabolism. Science, 320: 1504-1506.

Ni, M. and Yu, J.-H. 2007. A novel regulator couples sporogenesis and trehalose biogenesis in Aspergillus nidulans. PLoS ONE 2(10):e970 doi:10.1371/journal.pone.0000970.

Mah, J.-H., and Yu, J.-H. 2006. Upstream and downstream regulation of asexual development in Aspergillus fumigatus. Eukaryotic Cell. 5: 1585-1595.

Yu, J.-H. 2006. Heterotrimeric G protein signaling and RGSs in Aspergillus nidulans. Journal of Microbiology 44: 145-154.