Anjon Audhya
Professor
Biomolecular Chemistry
Regulation of vesicle biogenesis and membrane transport during development
Address
1111 Highland Avenue, WIMR Tower 2
Education
Ph.D., University of California, San Diego, 2002, Postdoctoral Research: Ludwig Institute for Cancer Research, La Jolla, California
Lab Website
Department
Biomolecular Chemistry
Research Interests
Regulation of vesicle biogenesis and membrane transport during development
Research Fields
Disease Biology, Cell Biology Development, Genomics & Proteomics, Neuro & Behavioral Genetics, C. elegans, Human, mouse & rat
Biography
Research Description:
Our laboratory is committed to understanding the fundamental mechanisms by which membrane proteins, lipids, and other macromolecules are transported throughout eukaryotic cells. To do so, we take advantage of numerous interdisciplinary approaches, including genetics, biochemistry, structural biology, biophysics, molecular biology and high-resolution fluorescence and electron microscopy.
Additionally, we use a variety of experimental systems, ranging from simple animal models (e.g. Caenorhabditis elegans) to human induced pluripotent stem cells (iPSCs). We also aim to recapitulate individual steps of membrane transport in vitro, using recombinant proteins and chemically defined lipids. Our ultimate goal is to identify the regulatory pathways that control membrane deformation, which enable vesicle formation in the endosomal and secretory systems. Although basic research is the cornerstone of our program, we also seek to define pathomechanisms that underlie human disease, focusing on the impact of mutations in key trafficking components that lead to cancer, neurodegeneration, asthma, and diabetes.
Representative Publications:
Kasberg, W., Luong, P., Swift, K.A., and Audhya, A. (2023) Nutrient deprivation alters the rate of COPII coat assembly to tune secretory protein transport. Nat. Commun. 14: 8140.
Clarke, A.L., Lettman, M.M., and Audhya, A. (2022) Lgd regulates ESCRT-III polymer assembly at multivesicular endosomes to control intralumenal vesicle formation. Mol. Biol. Cell. 33:ar144.
Peotter, J.L.,* Pustova, I.*, Lettman, M.M.*, Shatadal, S., Bradberry, M.M., Winter-Reed, A.D., Charan, M., Sharkey, E.E., Alvin, J.R., Bren, A.M., Ole, A.K., Chapman, E.R., Salamat, M.S., and Audhya, A. (2022) TFG regulates secretory and endosomal sorting pathways in neurons to promote their activity and maintenance. Proc. Natl. Acad. Sci. USA. 119:e2210649119.