Position title: Associate Professor
Regulation of vesicle biogenesis and membrane transport during development
- 5214A Biochemical Sciences Bldg
- Ph.D., University of California, San Diego, 2002, Postdoctoral Research: Ludwig Institute for Cancer Research, La Jolla, California
- Lab Website
- Biomolecular Chemistry
- Research Interests
- Regulation of vesicle biogenesis and membrane transport during development
- Research Fields
- Disease Biology, Cell Biology Development, Genomics & Proteomics, Neuro & Behavioral Genetics, C. elegans, Human, mouse & rat
Our laboratory is committed to understanding the fundamental mechanisms by which membrane proteins, lipids, and other macromolecules are transported throughout eukaryotic cells. To do so, we take advantage of numerous interdisciplinary approaches, including genetics, biochemistry, structural biology, biophysics, molecular biology and high-resolution fluorescence and electron microscopy.
Additionally, we use a variety of experimental systems, ranging from simple animal models (e.g. Caenorhabditis elegans) to human induced pluripotent stem cells (iPSCs). We also aim to recapitulate individual steps of membrane transport in vitro, using recombinant proteins and chemically defined lipids. Our ultimate goal is to identify the regulatory pathways that control membrane deformation, which enable vesicle formation in the endosomal and secretory systems. Although basic research is the cornerstone of our program, we also seek to define pathomechanisms that underlie human disease, focusing on the impact of mutations in key trafficking components that lead to cancer, neurodegeneration, asthma, and diabetes.
Schuh, A.L., Hanna, M., Quinney, K., Wang, L., Sarkeshik, A., Yates, J.R., and Audhya, A. (2015) The VPS-20 Subunit of the Endosomal Sorting Complex ESCRT-III Exhibits an Open Conformation in the Absence of Upstream Activation. Biochem. J. 466: 625-637.
Johnson, A., Bhattacharya, N., Hanna, M., Pennington, J.G., Schuh, A.L., Wang, L., Otegui, M.S., Stagg, S.M., and Audhya, A. (2015) TFG clusters COPII-coated transport carriers and promotes early secretory pathway organization. EMBO J. 34: 811-827.
Takahashi, H., Mayers, J.R., Wang, L., Edwardson, J.M., and Audhya, A. (2015) Hrs and STAM function synergistically to bind ubiquitin-modified cargoes in vitro. Biophys. J. 108: 76-84.