Jeff Hardin

Research Description:
We use the C. elegans embryo as a model for investigating cell movement and cell adhesion during embryonic development; understanding how cells move, and how they make and break adhesions has important implications for understanding birth defects during human development and for understanding cancer progression.

Representative Publications:
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Le*, S., Yu*, M., Fu*, C., Heier, J.A., Martin, S., Hardin, J., and Yan, J. (2024). Single-molecule force spectroscopy reveals intra- and intermolecular interactions of C. elegans HMP-1 during mechanotransduction. Proc. Nat. Acad. Sci. USA 2024 Sep 10;121(37):e2400654121. (*= co-first authors)

*Zhu, Y., *Tesone, Z., Tan, M., and Hardin, J. (2024). TIAM-1 regulates polarized protrusions during dorsal intercalation in the C. elegans embryo through both its GEF and N-terminal domains. J. Cell Sci. 137(5):jcs261509. (*co-first authors)

Martin, S.C.T., Qadota, H., Oberhauser, A.F., Hardin, J., and Benian, G.M. (2023). FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans. Mol. Biol. Cell 34:ar86, 1–13, August 1, 2023.

Serre, J.M. Mark M. Slabodnick, M.S., Goldstein, B. and Hardin, J. (2023). SRGP-1/srGAP and AFD-1/Afadin stabilize HMP-1/α-Catenin at rosettes to seal internalization sites following gastrulation in C. elegans. PLoS Genet. 2023 Mar 3;19(3):e1010507. doi: 10.1371/journal.pgen.1010507.

Lynch, A.M.*, Zhu, Y.*, Lucas, B.G., Winkelman, J.D., Bai, K., Martin, S.C.T. , Samuel Block, S., Slabodnick, M.S., Audhya, A., Goldstein, B., Pettitt, J., Gardel, M.L., and Hardin, J.(2022). TES-1/Tes and ZYX-1/Zyxin protect junctional actin networks under tension during epidermal morphogenesis in the C. elegans embryo. Curr. Biol 32, 5189–5199 (*= co-first authors).

Serre, J.M., Lucas, B., Martin, S.C.M. , Heier, J.A., Xiangqiang Shao, X., and Jeff Hardin, J. (2022). C. elegans srGAP is an α-catenin M domain-binding protein that strengthens cadherin-dependent adhesion during morphogenesis. Development 149 (18): dev200775.

Shao, X., Lucas, B.G., Strauch, J. and Hardin, J. (2019). The adhesion modulation domain of C. elegans α-catenin regulates actin binding during morphogenesis. Mol. Biol. Cell 30:2115-2123. PubMed

Choi, H.-J.*, Loveless, T.*, Lynch, A.M., Bang, I., Hardin, J+, and Weis, W.I.+. A conserved phosphorylation switch controls the interaction between cadherin and β-catenin In vitro and In vivo. Developmental Cell 33, 82–93. [*Co-first authors; +Co-senior authors]

Walck-Shannon, E. and Hardin, J. (2014). Cell intercalation from top to bottom. Nature Rev. Mol. Cell. Bio 15:34-48.

Maiden, S.L., Harrison, N., Keegan, J., Cain, B., Lynch, A.M., Pettitt, J., and Hardin, J. (2013). Specific conserved C-terminal amino acids of Caenorhabditis elegans HMP-1/α-catenin modulate F-actin binding independently of vinculin. J. Biol. Chem. 288:5694-5706.

Lynch, A.M., Grana, T., Cox-Paulson, E., Couthier, A., Cameron, M., Chin-Sang, I., Pettitt, J., and Hardin, J. (2012). A genome-wide functional screen identifies MAGI-1 as an L1CAM-dependent stabilizer of apical junctions in C. elegans. Curr. Biol. 22, 1891–1899.

Cox-Paulson, E., Walck-Shannon, E., Lynch, A., Yamashiro, S., Zaidel-Bar, R., Celeste C. Eno, C., Ono, S., and Hardin, J. (2012). Tropomodulin protects α-catenin-dependent junctional actin networks under stress during epithelial morphogenesis. Curr. Biol. 22:1500-1505.

Ikegami, R., Simokat, K., Zheng, H., Dixon, L., Garriga, G., Hardin, J. and Culotti, J. (2012). Semaphorin and Eph receptor signaling guide a series of cell movements for ventral enclosure in C. elegans. Curr. Biol. 22:1–11.

Loveless, T. and Hardin, J. (2012). Cadherin complexity: recent insights into cadherin superfamily function in C. elegans. Curr. Opin. Cell Biol. 24:695-701.

Zaidel-Bar, R., Joyce, M.J., Lynch, A.M., Witte, K., Audhya, A., and Hardin, J. (2010). The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogenesis. J. Cell Biol. 191, 761-9.

Kwiatkowski, A.V., Maiden, S.L., Pokutta, S., Choi, H.-J., Benjamin, J.M., Lynch, A.M., Nelson, W.J., Weis, W.I., and Hardin, J. (2010). In vitro and in vivo reconstitution of the cadherin-catenin-actin complex from Caenorhabditis elegans. PNAS 107:14591-14596.

Grana, T.M., Cox, E.A., Lynch, A.M., and Hardin, J. (2010). SAX-7/L1CAM and HMR-1/cadherin function redundantly in blastomere compaction and non-muscle myosin accumulation. Dev. Biol. 344:731–744.