Rupa Sridharan

Position title: Associate Professor


Phone: 608-316-4422

Cell and Regenerative Biology
Epigenetics: histone modification crosstalk and RNA processing,
Development and differentiation: from mouse and human stem cells
Genomics: Single cell transcriptomics and epigenomics

2118 Wisconsin Institutes for Discovery
Ph.D., University of California, Los Angeles (2006), Postdoctoral Research: Broad Stem Cell Institute, UCLA
Lab Website
Cell and Regenerative Biology
Research Interests
Epigenetics: histone modification crosstalk and RNA processing, Development and differentiation: from mouse and human stem cells, Genomics: Single cell transcriptomics and epigenomics
Research Fields
Cell Biology, Computational, Systems, and Synthetic Biology, Development, Gene Expression, Genomics and Proteomics, Mouse and Other Mammals

Research Description:

Our lab is focused on investigating how chromatin modifications that control gene expression and be transmitted across cell divisions control the establishment, maintainence and disruption of cell identity in development and disease. For this purpose we use genomic and gene editing techniques wide techniques to query the epigenome and transcriptome at the population and single cell level. Ultimately we are interested in gaining fundamental insights into cell fate and applying the information to derive cells with desired properties for regenerative therapy.

Representative Publications:
Search PubMed for more publications by Rupa Sridharan

Roy, S. * and Sridharan, R. *(2017) Chromatin module inference on cellular trajectories reveals poised epigenetic states in reprogramming to pluripotency. Genome Research 27: 1250-1262 * co-corresponding author.

Tran, K.A., Pietrzak, S.P, Zaidan, N.Z., Siahpirani, A.F., McKalla S.G., Iyer, G., Roy,S., and Sridharan, R. (2019) Defining reprogramming checkpoints from single-cell analysis of induced pluripotency Cell Reports 27(6):1726-1741

Tran, K.A., Dillingham, C.D. and Sridharan, R. (2019) Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity EMBO J- e101681. doi: 10.15252/embj.2019101681

Zaidan, N.Z., and Sridharan, R. (2020) HP1 gamma regulates H3K36 methylation and pluripotency in embryonic stem cells. Nucleic Acids Res. 2020 16;48(22):12660-12674. doi: 10.1093/nar/gkaa1091. PMID: 33237287; PMCID: PMC7736818.

Wille, C.K. and Sridharan, R. (2022) DOT1L inhibition enhances pluripotency beyond acquisition of epithelial identity and without immediate suppression of the somatic transcriptome. Stem Cell Reports. 2022 Feb 8;17(2):384-396. PMID: 34995500.