Cell and Regenerative Biology
Epigenetics and Induced pluripotent stem cells
- 2118 Wisconsin Institutes for Discovery
- Ph.D., University of California, Los Angeles (2006), Postdoctoral Research: Broad Stem Cell Institute, UCLA
- Lab Website
- Cell and Regenerative Biology
- Research Interests
- Epigenetics and Induced pluripotent stem cells
- Research Fields
- Development, Gene Expression, Genomics & Proteomics, Human, mouse & rat
Embryonic stem (ES) cells have the ability to divide indefinitely and to differentiate into any tissue under the correct set of chemical stimuli. Transcription factor- mediated reprogramming, initially demonstrated in mouse somatic cells, is the process by which the overexpression of a few transcription factors, usually, Oct4, Sox2, c-Myc and Klf4 converts differentiated cells into induced pluripotent stem (iPS) cells. Multiple molecular and functional studies have shown that iPS cells are highly similar to ES cells. Human somatic cells can also be reprogrammed, providing iPS cells both as tools for translational research such as for in vitro drug screens and for cell replacement therapy. Only about 1 % of cells complete the reprogramming process suggesting that multiple barriers have to be overcome for this dramatic change in cell fate to occur. Research in the lab will be focused on understanding the epigenetic roadblocks to the reprogramming process to illuminate both the mechanisms that control pluripotency and the stability of the differentiated state.
Topics of interest include:
Transcription factor mediated reprogramming
Histone post-translational modification changes
Cell fate maintenance
Search PubMed for more publications by Rupa Sridharan
Tran, K.A., Jackson, S.A., Olufs, Z.P.G., Zaidan, N.Z., Leng, N., Kendziorski, C., Roy, S., and Sridharan, R. (2015) Collaborative rewiring of the pluripotency network by chromatin and signaling modulating pathways. Nat. Commun. 6:6188 doi: 10.1038/ncomms7188
Jackson, S.A. and Sridharan, R. (2013) Peering into the black box of reprogramming to the pluripotent state. Curr Pathobiol Rep, 1,129-136.
Jackson, S.A. and Sridharan, R. (2013) The nexus of Tet1 and the pluripotency network. Cell Stem Cell, 12, 387-88.
Sridharan, R., Gonzales-Cope, M., Chronis, C.,Bonora, G., McKee, R., Patel,S.,Lopez,D., Mishra, N.,Pellegrini, M., Carey, M., Garcia, B.A. and Plath, K. (2013) Proteomic and genomic approaches reveal critical functions of H3K9 methylation and Heterochromatin Protein-1γ in reprogramming to pluripotency. Nat. Cell. Bio. 15(7):872-82
Sridharan R *., Tchieu J *., Mason M.J. *. , Yachechko R., Kuoy E., Horvath S., Zhou Q. and Plath K. (2009). Role of the murine reprogramming factors in the induction of pluripotency. * authors contributed equally to this work. Cell 136 (2), 364-77
Maherali, N.*, Sridharan, R.*, Xie, W., Utikal, J., Eminli, S., Arnold, K., Stadtfeld, M., Yachechko, R., Tchieu. J., Jaenisch, R., Plath, K.#, and Hochedlinger, K.# (2007). Global epigenetic remodeling in directly reprogrammed fibroblasts. * both authors contributed equally to this work; # co-corresponding authors. Cell Stem Cell 1, 55-70
Lowry, W. E., Richter, L .,Yachechko, R., Pyle, A. D., Tchieu, J., Sridharan, R., Clark, A. T. and Plath, K.(2008) Generation of human induced pluripotent cells from dermal fibroblasts. PNAS 105, 2883-2888