Cell and Regenerative Biology
Epigenetics and Induced pluripotent stem cells
- 2118 Wisconsin Institutes for Discovery
- Ph.D., University of California, Los Angeles (2006), Postdoctoral Research: Broad Stem Cell Institute, UCLA
- Lab Website
- Cell and Regenerative Biology
- Research Interests
- Epigenetics in development and disease
- Research Fields
- Development, Gene Expression, Genomics & Proteomics, Human, mouse & rat
During the development of a multicellular organism a single cell can give rise to an entire organism made of a multitude of cells of specialized function. Such functional specification is determined both by the levels of expression of transcription factors and the receptivity of the chromatin state of the target genes to the binding of such factors. Once cell identity is established, epigenetic modifications, including those on histones and DNA, can ensure that it is stably maintained through cell divisions. Cell fate can be altered by the overexpression of proteins from other cell types. One of the most dramatic examples of such cell fate conversion is the reprogramming of unipotent somatic cells to induced pluripotent stem cells (iPSCs). iPSCs can self renew indefinitely and differentiate into all other cell types similar to embryonic stem cells (ESCs). Our lab is focused on investigating how cell identity is established from a pluripotent state and how it can be disrupted to generate a new cell fate. For this purpose we use genome wide techniques to query the epigenome and transcriptome at the population and single cell level.
Search PubMed for more publications by Rupa Sridharan
Zaidan, N.Z., Walker, K.J., Brown, J.E., Scalf, M., Shortreed, M., Smith, L.M., Iyer, G., and Sridharan, R. (2018) Compartmentalization of HP1 proteins in pluripotency acquisition and maintenance. Stem Cell Reports, 10 (2): 627–641.
Roy, S.* and Sridharan, R.* (2017) Chromatin module inference on cellular trajectories identifies key transition points and poised epigenetic states in diverse developmental processes. Genome Research 27: 1250–1262. *co-corresponding authors
Jackson, S.A. *, Olufs, Z.P.G. *, Tran, K.A., Zaidan, N.Z., and Sridharan, R. (2016) Alternative routes to induced pluripotent stem cells revealed by reprogramming of neural lineage. Stem Cell Reports 6 (3): 302–11. *co-first authors
Tran, K.A., Jackson, S.A., Olufs, Z.P.G., Zaidan, N.Z., Leng, N., Kendziorski, C., Roy, S., and Sridharan, R. (2015) Collaborative rewiring of the pluripotency network by chromatin and signaling modulating pathways. Nat. Commun., 6: 6188. doi: 10.1038/ncomms7188
Jackson, S.A. and Sridharan, R. (2013) The nexus of Tet1 and the pluripotency network. Cell Stem Cell, 12, 387-88.