Rupa Sridharan
Position title: Associate Professor
Email: rsridharan2@wisc.edu
Phone: 608-316-4422
Address:
Cell and Regenerative Biology
Epigenetics: histone modification crosstalk and RNA processing,
Development and differentiation: from mouse and human stem cells
Genomics: Single cell transcriptomics and epigenomics
- Address
- 330 N.Orchard Street Madison WI-53715, 2118
- Education
- Ph.D., University of California, Los Angeles (2006), Postdoctoral Research: Broad Stem Cell Institute, UCLA
- Lab Website
- https://sridharanlab.discovery.wisc.edu
- Department
- Cell and Regenerative Biology
- Research Interests
- Epigenetics, Stem Cells, Development
- Research Fields
- Cell Biology, Computational, Systems, and Synthetic Biology, Development, Gene Expression, Genomics and Proteomics, Mouse and Other Mammals
Research Description:
Our lab is focused on investigating how chromatin modifications that control gene expression and can be transmitted across cell divisions control the establishment , maintainence and disruption of cell identity in development and disease. For this purpose we use genomic and gene editing techniques wide techniques to query the epigenome and transcriptome at the population and single cell level. Ultimately we are interested in gaining fundamental insights into cell fate specification and applying the information to derive cells with desired properties for regenerative therapy.
Representative Publications:
Search PubMed for more publications by Rupa Sridharan
Tran, K.A., Pietrzak, S.P, Zaidan, N.Z., Siahpirani, A.F., McKalla S.G., Iyer, G., Roy,S., and Sridharan, R. (2019) Defining reprogramming checkpoints from single-cell analysis of induced pluripotency Cell Reports 27(6):1726-1741
Tran, K.A., Dillingham, C.M., and Sridharan R. (2019) Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity. EMBO J. e101681. doi: 10.15252/embj.2019101681
Wille, C.K., Zhang, X., Haws, S.A., Denu, J.M. and Sridharan, R. (2023) DOT1L is a barrier to histone acetylation during reprogramming to pluripotency. Science Advances Nov 15;9(46):eadf3980. doi: 10.1126/sciadv.adf3980 PMID: 37976354; PMCID: PMC10656071.
Zhang, S., Pyne, S., Pietrzak, S., Halberg, S., McCalla, S.G., Siahpirani, A.F., Sridharan, R., Roy, S. Inference of cell type-specific gene regulatory networks on cell lineages from single cell omic datasets. Nat Commun. 2023 May 27;14(1):3064.PMID: 37244909; PMCID: PMC10224950.
Roy, S. * and Sridharan, R. *(2017) Chromatin module inference on cellular trajectories reveals poised epigenetic states in reprogramming to pluripotency. Genome Research 27: 1250-1262 * co-corresponding author.
Tran, K.A., Pietrzak, S.P, Zaidan, N.Z., Siahpirani, A.F., McKalla S.G., Iyer, G., Roy,S., and Sridharan, R. (2019) Defining reprogramming checkpoints from single-cell analysis of induced pluripotency Cell Reports 27(6):1726-1741
Tran, K.A., Dillingham, C.D. and Sridharan, R. (2019) Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity EMBO J- e101681. doi: 10.15252/embj.2019101681
Zaidan, N.Z., and Sridharan, R. (2020) HP1 gamma regulates H3K36 methylation and pluripotency in embryonic stem cells. Nucleic Acids Res. 2020 16;48(22):12660-12674. doi: 10.1093/nar/gkaa1091. PMID: 33237287; PMCID: PMC7736818.
Wille, C.K. and Sridharan, R. (2022) DOT1L inhibition enhances pluripotency beyond acquisition of epithelial identity and without immediate suppression of the somatic transcriptome. Stem Cell Reports. 2022 Feb 8;17(2):384-396. PMID: 34995500.